FRS2 Recombinant Rabbit Monoclonal Antibody [JE30-77]
cat.: HA721434
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE30-77 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FRS2 aa 91-240 / 508. |
| Positive control: | HeLa cell lysate, A549 cell lysate, HEK-293 cell lysate, NCI-H441 cell lysate, MCF7 cell lysate, HeLa. |
| Subcellular location: | Endomembrane system. |
| Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q8WU20 Human |
| Alternative names: | FGFR signaling adaptor SNT FGFR signalling adaptor FGFR substrate 2 FGFR-signaling adaptor SNT Fibroblast growth factor receptor substrate 2 FRS 2 FRS2 FRS2_HUMAN FRS2A FRS2alpha SNT 1 SNT SNT-1 SNT1 Suc 1 Associated Neurotrophic Factor Target Suc1 associated neurotrophic factor target 1 Suc1-associated neurotrophic factor target 1 |
Images
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Fig1:
Western blot analysis of FRS2 on different lysates with Rabbit anti-FRS2 antibody (HA721434) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NCI-H441 cell lysate Lane 5: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 70 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721434) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HeLa cells labeling FRS2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721434, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.