FRS2 Recombinant Rabbit Monoclonal Antibody [JE30-77]
cat.: HA721434
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, FC
Clonality: Monoclonal
Clone number: JE30-77
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 57 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human FRS2 aa 91-240 / 508.
Positive control: HeLa cell lysate, A549 cell lysate, HEK-293 cell lysate, NCI-H441 cell lysate, MCF7 cell lysate, HeLa.
Subcellular location: Endomembrane system.
Recommended Dilutions:
  WB
  FC

1:1,000
1:500-1:1,000
Uniprot #: SwissProt: Q8WU20 Human
Alternative names: FGFR signaling adaptor SNT FGFR signalling adaptor FGFR substrate 2 FGFR-signaling adaptor SNT Fibroblast growth factor receptor substrate 2 FRS 2 FRS2 FRS2_HUMAN FRS2A FRS2alpha SNT 1 SNT SNT-1 SNT1 Suc 1 Associated Neurotrophic Factor Target Suc1 associated neurotrophic factor target 1 Suc1-associated neurotrophic factor target 1
Images
HA721434_1.jpg Fig1: Western blot analysis of FRS2 on different lysates with Rabbit anti-FRS2 antibody (HA721434) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: NCI-H441 cell lysate
Lane 5: MCF7 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 57 kDa
Observed band size: 70 kDa

Exposure time: 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721434) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721434_2.jpg Fig2: Flow cytometric analysis of HeLa cells labeling FRS2.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721434, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.