Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB, FC |
Clonality: | Monoclonal |
Clone number: | JE30-77 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 57 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human FRS2 aa 91-240 / 508. |
Positive control: | HeLa cell lysate, A549 cell lysate, HEK-293 cell lysate, NCI-H441 cell lysate, MCF7 cell lysate, HeLa. |
Subcellular location: | Endomembrane system. |
Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
Uniprot #: | SwissProt: Q8WU20 Human |
Alternative names: | FGFR signaling adaptor SNT FGFR signalling adaptor FGFR substrate 2 FGFR-signaling adaptor SNT Fibroblast growth factor receptor substrate 2 FRS 2 FRS2 FRS2_HUMAN FRS2A FRS2alpha SNT 1 SNT SNT-1 SNT1 Suc 1 Associated Neurotrophic Factor Target Suc1 associated neurotrophic factor target 1 Suc1-associated neurotrophic factor target 1 |
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Fig1:
Western blot analysis of FRS2 on different lysates with Rabbit anti-FRS2 antibody (HA721434) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HEK-293 cell lysate Lane 4: NCI-H441 cell lysate Lane 5: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 70 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721434) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Flow cytometric analysis of HeLa cells labeling FRS2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721434, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |