Tbp7 Recombinant Rabbit Monoclonal Antibody [JE62-72]
cat.: HA721435
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Monoclonal
Clone number: JE62-72
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 47 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Tbp7 aa 369-418.
Positive control: K-562 cell lysate, HEK-293 cell lysate, HeLa cell lysate, U-2 OS cell lysate, HepG2 cell lysate, MCF7 cell lysate, Jurkat cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human colon carcinoma tissue, mouse cerebellum tissue, rat cerebellum tissue, U-2 OS.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:1,000-1:2,000
1:1,000-1:2,000
1:100
1:500-1:1,000
Uniprot #: SwissProt: P43686 Human | P54775 Mouse | Q63570 Rat
Alternative names: 26S protease regulatory subunit 6B 26S proteasome AAA ATPase subunit RPT3 26S proteasome AAA-ATPase subunit RPT3 MB67 interacting protein MB67-interacting protein MIP224 Protease 26S subunit 6 Proteasome (prosome macropain) 26S subunit ATPase 4 Proteasome 19S S6 Proteasome 26S subunit ATPase 4 Proteasome 26S subunit, ATPase, 4 PRS6B_HUMAN PSMC4 RPT3 S6 Tat binding protein 7 TAT-binding protein 7 TBP 7 TBP-7
Images
HA721435_1.jpg Fig1: Western blot analysis of Tbp7 on different lysates with Rabbit anti-Tbp7 antibody (HA721435) at 1/1,000 dilution.

Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: U-2 OS cell lysate (20 µg/Lane)
Lane 5: HepG2 cell lysate (20 µg/Lane)
Lane 6: MCF7 cell lysate (20 µg/Lane)
Lane 7: Jurkat cell lysate (20 µg/Lane)
Lane 8: Mouse testis tissue lysate (40 µg/Lane)
Lane 9: Rat testis tissue lysate (40 µg/Lane)

Predicted band size: 47 kDa
Observed band size: 47 kDa

Exposure time: 17 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721435) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721435_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Tbp7 antibody (HA721435) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721435) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721435_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Tbp7 antibody (HA721435) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721435) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721435_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Tbp7 antibody (HA721435) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721435) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721435_5.jpg Fig5: Immunocytochemistry analysis of U-2 OS cells labeling Tbp7 with Rabbit anti-Tbp7 antibody (HA721435) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tbp7 antibody (HA721435) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721435_6.jpg Fig6: Flow cytometric analysis of U-2 OS cells labeling Tbp7.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721435, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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