Fetuin A Recombinant Rabbit Monoclonal Antibody [JE54-96]
cat.: HA721436
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE54-96 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human AHSG aa 19-68. |
| Positive control: | Human placenta tissue lysates, human liver tissue, human liver carcinoma tissue, HepG2. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P02765 Human |
| Alternative names: | 59 kDa bone sialic acid containing protein A2HS Aa2 066 AHS Ahsg Alpha 2 HS glycoprotein Alpha 2 HS glycoprotein chain A Alpha 2 HS glycoprotein chain B Alpha 2 Z globulin Alpha 2HS glycoprotein Alpha-2-HS-glycoprotein chain B Alpha-2-Z-globulin alpha2 Heremans Schmid glycoprotein Asialofetuin Ba alpha 2 glycoprotein Ba-alpha-2-glycoprotein BSP Countertrypin FETUA FETUA_HUMAN Fetuin A Fetuin, mouse, homolog of Fetuin-A FetuinA Glycoprotein PP63 HSGA pp63 16G2 2310011O17Rik AI255764 D17980 Fet Fetub FETUB_HUMAN Fetuin beta Fetuin-B Fetuin-like protein Fetuin-like protein IRL685 Gugu IRL685 Pp63 |
Images
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Fig1:
Western blot analysis of Fetuin A on human placenta tissue lysates with Rabbit anti-Fetuin A antibody (HA721436) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time: 5 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721436) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Fetuin A antibody (HA721436) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721436) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Fetuin A antibody (HA721436) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721436) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of HepG2 cells labeling Fetuin A. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721436, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.