Palladin Recombinant Rabbit Monoclonal Antibody [JE66-56]
cat.: HA721440
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE66-56 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 151 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Palladin aa 1-100 / 1,383. |
| Positive control: | HeLa cell lysate, SiHa cell lysate, A431 cell lysate, SW480 cell lysate, 293T cell lysate, HEK-293 cell lysate, MDA-MB-231 cell lysate, MDA-MB-468 cell lysate, MCF7 cell lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton, Cytoplasm, myofibril, sarcomere, Z line. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q8WX93 Human |
| Alternative names: | CGI 151 CGI151 KIAA0992 MYN Myoneurin pa Palladin cytoskeletal associated protein Palld PNCA1 Sarcoma antigen NY SAR 77 SIH002 Stx13bp1 |
Images
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Fig1:
Western blot analysis of Palladin on different lysates with Rabbit anti-Palladin antibody (HA721440) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15µg/Lane) Lane 2: SiHa cell lysate (10µg/Lane) Lane 3: A431 cell lysate (15 µg/Lane) Lane 4: SW480 cell lysate (30 µg/Lane) Lane 5: 293T cell lysate (20 µg/Lane) Lane 6: HEK-293 cell lysate (20 µg/Lane) Lane 7: MDA-MB-231 cell lysate (20 µg/Lane) Lane 8: MDA-MB-468 cell lysate (20 µg/Lane) Lane 9: MCF7 cell lysate (20 µg/Lane) Predicted band size: 151 kDa Observed band size: 110/151 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721440) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.