Parathyroid Hormone Recombinant Rabbit Monoclonal Antibody [JE31-67]
cat.: HA721441
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE31-67
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 13 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Parathyroid Hormone aa 26-75 / 115.
Positive control: Jurkat cell lysate, mouse thymus tissue lysate, rat thymus tissue lysate.
Subcellular location: Secreted.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P01270 Human | P04089 Rat
Entrez Gene: 19226 Mouse
Alternative names: hPTH Parathormone Parathyrin Parathyroid hormone 1 Parathyroid hormone Prepro PTH Preproparathyroid hormone PTH PTH1 PTH1 receptor PTH1R PTHR PTHR1 PTHY_HUMAN
Images
HA721441_1.jpg Fig1: Western blot analysis of Parathyroid Hormone on different lysates with Rabbit anti-Parathyroid Hormone antibody (HA721441) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate (15 µg/Lane)
Lane 2: Mouse thymus tissue lysate (30 µg/Lane)
Lane 3: Rat thymus tissue lysate (30 µg/Lane)

Predicted band size: 13 kDa
Observed band size: 16 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721441) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.