Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE31-67 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 13 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human Parathyroid Hormone aa 26-75 / 115. |
Positive control: | Jurkat cell lysate, mouse thymus tissue lysate, rat thymus tissue lysate. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: P01270 Human | P04089 Rat Entrez Gene: 19226 Mouse |
Alternative names: | hPTH Parathormone Parathyrin Parathyroid hormone 1 Parathyroid hormone Prepro PTH Preproparathyroid hormone PTH PTH1 PTH1 receptor PTH1R PTHR PTHR1 PTHY_HUMAN |
Fig1:
Western blot analysis of Parathyroid Hormone on different lysates with Rabbit anti-Parathyroid Hormone antibody (HA721441) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (15 µg/Lane) Lane 2: Mouse thymus tissue lysate (30 µg/Lane) Lane 3: Rat thymus tissue lysate (30 µg/Lane) Predicted band size: 13 kDa Observed band size: 16 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721441) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |