ATG10 Recombinant Rabbit Monoclonal Antibody [JE59-86]
cat.: HA721444
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE59-86 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to Human ATG10 aa 180-220 (C terminal) |
| Positive control: | HeLa cell lysate, 293T cell lysate, K-562 cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9H0Y0 Human |
| Alternative names: | Apg 10 APG 10L APG10 APG10 autophagy 10 like APG10 like APG10, S. cerevisiae, homolog of APG10-like APG10L ATG 10 ATG10 ATG10 autophagy related 10 homolog (S. cerevisiae) ATG10 autophagy related 10 homolog ATG10_HUMAN autophagy 10, S. cerevisiae, homolog of Autophagy related protein 10 Autophagy-related protein 10 DKFZP586I0418 FLJ13954 pp12616 Ubiquitin-like-conjugating enzyme ATG10 |
Images
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Fig1:
Western blot analysis of ATG10 on different lysates with Rabbit anti-ATG10 antibody (HA721444) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: K-562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 25 kDa Observed band size: 30 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721444) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.