MAD1L1 Recombinant Rabbit Monoclonal Antibody [JE35-54]
cat.: HA721446
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE35-54 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MAD1L1 aa 31-180 / 718. |
| Positive control: | HCT 116 cell lysate, HeLa cell lysate, U-2 OS cell lysate, Jurkat cell lysate, MCF7 cell lysate, A431 cell lysate, A375 cell lysate. |
| Subcellular location: | Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9Y6D9 Human |
| Alternative names: | hMAD1 HsMAD1 MAD1 MAD1 mitotic arrest deficient like 1 (yeast) MAD1-like protein 1 MAD1L1 MD1L1_HUMAN Mitotic arrest deficient 1 Mitotic arrest deficient 1-like protein 1 Mitotic checkpoint MAD1 protein homolog Mitotic spindle assembly checkpoint protein MAD1 PIG9 Tax binding protein 181 Tax-binding protein 181 TP53I9 Tumor protein p53 inducible protein 9 TXBP181 |
Images
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Fig1:
Western blot analysis of MAD1L1 on different lysates with Rabbit anti-MAD1L1 antibody (HA721446) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HeLa cell lysate Lane 3: U-2 OS cell lysate Lane 4: Jurkat cell lysate Lane 5: MCF7 cell lysate Lane 6: A431 cell lysate Lane 7: A375 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721446) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.