CABP1 Recombinant Rabbit Monoclonal Antibody [JE56-72]
cat.: HA721448
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE56-72 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CABP aa 150-250. |
| Positive control: | U-87 MG cell lysate, human brain tissue lysate, U-87 MG. |
| Subcellular location: | Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, Synapse. |
| Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9NZU7 Human |
| Alternative names: | CABP 1 CaBP1 CABP1_HUMAN Calbrain Calcium binding protein 1 Calcium binding protein 5 Calcium-binding protein 1 Caldendrin HCALB BR HCALB_BR |
Images
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Fig1:
Western blot analysis of CABP1 on different lysates with Rabbit anti-CABP1 antibody (HA721448) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate (20 µg/Lane) Lane 2: Human brain tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721448) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of U-87 MG cells labeling CABP1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721448, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.