SHB Recombinant Rabbit Monoclonal Antibody [JE57-12]
cat.: HA721449
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE57-12 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SHB aa 450-509. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, K-562 cell lysate, HEK-293 cell lysate, A549. |
| Subcellular location: | Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q15464 Human |
| Alternative names: | bA3J10.2 OTTHUMP00000021397 RP11 3J10.8 RP113J10.8 SH2 domain containing adapter protein B SH2 domain-containing adapter protein B SHB (Src homology 2 domain containing) adaptor protein B SHB adaptor protein (a Src homology 2 protein) SHB adaptor protein Shb SHB_HUMAN Src homology 2 domain containing adaptor protein B Src homology 2 protein |
Images
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Fig1:
Western blot analysis of SHB on different lysates with Rabbit anti-SHB antibody (HA721449) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF7 cell lysate Lane 4: K-562 cell lysate Lane 5: HEK-293 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721449) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of A549 cells labeling SHB. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721449, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.