HA721450

SMC2 Recombinant Rabbit Monoclonal Antibody [PSH0-62]

cat.: HA721450

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Monkey
Applications:	WB, IHC-P, IF-Cell
Clonality:	Monoclonal
Clone number:	PSH0-62

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 136 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human SMC2 aa 451-750 / 1,197.
Positive control:	HeLa cell lysate, HEK-293 cell lysate, HT-29 cell lysate, HepG2 cell lysate, A431 cell lysate, SH-SY5Y cell lysate, Jurkat cell lysate, Raji cell lysate, COS-1 cell lysate, human colon carcinoma tissue, human colon tissue, human colon lymph nodes tissue, HeLa, HT-29.
Subcellular location:	Nucleus, Cytoplasm, Chromosome.
Recommended Dilutions: WB IHC-P IF-Cell	1:1,000 1:1,000 1:100
Uniprot #:	SwissProt: O95347 Human
Alternative names:	CAP E CAPE Chromosome associated protein E Chromosome-associated protein E FLJ10093 hCAP E hCAP-E hCAPE PRO0324 SMC 2 SMC protein 2 SMC-2 SMC2 SMC2 L1 SMC2_HUMAN SMC2L1 Structural maintenance of chromosomes (SMC) family member chromosome associated protein E Structural maintenance of chromosomes 2 Structural maintenance of chromosomes 2 like 1 Structural maintenance of chromosomes 2 yeast like 1 Structural maintenance of chromosomes protein 2 XCAP E homolog XCAP-E homolog XCAPE homolog

Images

Fig1: Western blot analysis of SMC2 on different lysates with Rabbit anti-SMC2 antibody (HA721450) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (30 µg/Lane)
Lane 2: HEK-293 cell lysate (30 µg/Lane)
Lane 3: HT-29 cell lysate (30 µg/Lane)
Lane 4: HepG2 cell lysate (30 µg/Lane)
Lane 5: A431 cell lysate (30 µg/Lane)
Lane 6: SH-SY5Y cell lysate (30 µg/Lane)
Lane 7: Jurkat cell lysate (30 µg/Lane)
Lane 8: Raji cell lysate (30 µg/Lane)
Lane 9: COS-1 cell lysate (24 µg/Lane)

Predicted band size: 136 kDa
Observed band size: 136 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721450) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SMC2 antibody (HA721450) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721450) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-SMC2 antibody (HA721450) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721450) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human colon lymph nodes tissue with Rabbit anti-SMC2 antibody (HA721450) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721450) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunocytochemistry analysis of HeLa cells labeling SMC2 with Rabbit anti-SMC2 antibody (HA721450) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMC2 antibody (HA721450) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Fig6: Immunocytochemistry analysis of HT-29 cells labeling SMC2 with Rabbit anti-SMC2 antibody (HA721450) at 1/100 dilution.

Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMC2 antibody (HA721450) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.