SMC2 Recombinant Rabbit Monoclonal Antibody [PSH0-63]
cat.: HA721451
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Monkey |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH0-63 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 136 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SMC2 aa 451-750 / 1,197. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, HT-29 cell lysate, HepG2 cell lysate, A431 cell lysate, SH-SY5Y cell lysate, Jurkat cell lysate, Raji cell lysate, COS-1 cell lysate, human colon carcinoma tissue, HeLa, HT-29. |
| Subcellular location: | Nucleus, Cytoplasm, Chromosome. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:1,000 1:100 |
| Uniprot #: | SwissProt: O95347 Human |
| Alternative names: | CAP E CAPE Chromosome associated protein E Chromosome-associated protein E FLJ10093 hCAP E hCAP-E hCAPE PRO0324 SMC 2 SMC protein 2 SMC-2 SMC2 SMC2 L1 SMC2_HUMAN SMC2L1 Structural maintenance of chromosomes (SMC) family member chromosome associated protein E Structural maintenance of chromosomes 2 Structural maintenance of chromosomes 2 like 1 Structural maintenance of chromosomes 2 yeast like 1 Structural maintenance of chromosomes protein 2 XCAP E homolog XCAP-E homolog XCAPE homolog |
Images
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Fig1:
Western blot analysis of SMC2 on different lysates with Rabbit anti-SMC2 antibody (HA721451) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 µg/Lane) Lane 2: HEK-293 cell lysate (30 µg/Lane) Lane 3: HT-29 cell lysate (30 µg/Lane) Lane 4: HepG2 cell lysate (30 µg/Lane) Lane 5: A431 cell lysate (30 µg/Lane) Lane 6: SH-SY5Y cell lysate (30 µg/Lane) Lane 7: Jurkat cell lysate (30 µg/Lane) Lane 8: Raji cell lysate (30 µg/Lane) Lane 9: COS-1 cell lysate (24 µg/Lane) Predicted band size: 136 kDa Observed band size: 136 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721451) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SMC2 antibody (HA721451) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721451) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of HeLa cells labeling SMC2 with Rabbit anti-SMC2 antibody (HA721451) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMC2 antibody (HA721451) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunocytochemistry analysis of HT-29 cells labeling SMC2 with Rabbit anti-SMC2 antibody (HA721451) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SMC2 antibody (HA721451) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.