Pericentrin Recombinant Rabbit Monoclonal Antibody [PSH0-66]
cat.: HA721454
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH0-66 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 378 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human aa 201-600 / 3,336. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, K-562 cell lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O95613 Human |
| Alternative names: | Centrosome Marker Ken Kendrin KIAA0402 MOPD2 PCN PCNT 2 PCNT PCNT B PCNT_HUMAN PCNT1 PCNT2 PCNTB PCTN2 Pericentrin 1 Pericentrin 2 Pericentrin 380 Pericentrin Pericentrin B Pericentrin-B SCKL4 |
Images
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Fig1:
Western blot analysis of Pericentrin on different lysates with Rabbit anti-Pericentrin antibody (HA721454) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: MCF7 cell lysate Lane 4: K-562 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 378 kDa Observed band size: 220 kDa (cleavage form) Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721454) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.