CRBN Recombinant Rabbit Monoclonal Antibody [PSH0-69]
cat.: HA721457
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | PSH0-69 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CRBN aa 1-442 / 442. |
| Positive control: | HEK-293 cell lysate, A375 cell lysate, A549 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, NIH/3T3 cell lysate, MCF7 cell lysate, mouse brain tissue lysate, rat testis tissue lysate, human testis tissue, human brain tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Membrane. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000 1:200 1-2μg/sample |
| Uniprot #: | SwissProt: Q96SW2 Human | Q8C7D2 Mouse | Q56AP7 Rat |
| Alternative names: | 2610203G15Rik 2900045O07Rik AF229032 AW108261 Cereblon Crbn CRBN_HUMAN DKFZp781K0715 MGC27358 MRT2A OTTHUMP00000209555 piL Protein cereblon Protein x 0001 |
Images
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Fig1:
Western blot analysis of CRBN on different lysates with Rabbit anti-CRBN antibody (HA721457) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A375 cell lysate Lane 3: A549 cell lysate Lane 4: HeLa cell lysate Lane 5: SH-SY5Y cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: MCF7 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721457) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CRBN antibody (HA721457) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721457) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-CRBN antibody (HA721457) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721457) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Western blot analysis of CRBN on different lysates with Rabbit anti-CRBN antibody (HA721457) at 1/1,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si CRBN cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721457) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig5:
CRBN was immunoprecipitated in 0.2mg HeLa cell lysate with HA721457 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA721457 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA721457 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA721457 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 10 seconds; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.