CRBN Recombinant Rabbit Monoclonal Antibody [PSH0-70]
cat.: HA721458
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: PSH0-70
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Recombinant protein within human CRBN aa 1-442 / 442.
Positive control: HEK-293 cell lysate, A375 cell lysate, A549 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, rat testis tissue lysate, human testis tissue, mouse testis tissue, rat testis tissue.
Subcellular location: Cytoplasm, Nucleus, Membrane.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:200
Uniprot #: SwissProt: Q96SW2 Human | Q8C7D2 Mouse | Q56AP7 Rat
Alternative names: 2610203G15Rik 2900045O07Rik AF229032 AW108261 Cereblon Crbn CRBN_HUMAN DKFZp781K0715 MGC27358 MRT2A OTTHUMP00000209555 piL Protein cereblon Protein x 0001
Images
HA721458_1.jpg Fig1: Western blot analysis of CRBN on different lysates with Rabbit anti-CRBN antibody (HA721458) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: A375 cell lysate
Lane 3: A549 cell lysate
Lane 4: HeLa cell lysate
Lane 5: SH-SY5Y cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: Mouse brain tissue lysate
Lane 8: Rat testis tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 51 kDa
Observed band size: 51 kDa

Exposure time: 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721458) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721458_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CRBN antibody (HA721458) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721458) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721458_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-CRBN antibody (HA721458) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721458) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721458_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-CRBN antibody (HA721458) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721458) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721458_5.jpg Fig5: Western blot analysis of CRBN on different lysates with Rabbit anti-CRBN antibody (HA721458) at 1/1,000 dilution.

Lane 1: HeLa-si NT cell lysate
Lane 2: HeLa-si CRBN cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 51 kDa
Observed band size: 51 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721458) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.