LSD2 Recombinant Rabbit Monoclonal Antibody [JE32-40]
cat.: HA721462
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE32-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human LSD2 aa 71-120 / 822. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, K-562 cell lysate, MCF7 cell lysate, U-2 OS cell lysate, HCT 116 cell lysate. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q8NB78 Human |
| Alternative names: | amine oxidase (flavin containing) domain 1 amine oxidase, flavin containing 1 AOF1 bA204B7.3 C6orf193 dJ298J15.2 Flavin-containing amine oxidase domain-containing protein 1 FLJ33898 FLJ34109 FLJ43328 KDM1B KDM1B_HUMAN LSD2 lysine (K)-specific demethylase 1B Lysine-specific demethylase 2 Lysine-specific histone demethylase 1B Lysine-specific histone demethylase 2 |
Images
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Fig1:
Western blot analysis of LSD2 on different lysates with Rabbit anti-LSD2 antibody (HA721462) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lane 4: K-562 cell lysate Lane 5: MCF7 cell lysate Lane 6: U-2 OS cell lysate Lane 7: HCT 116 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721462) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of LSD2 on different lysates with Rabbit anti-LSD2 antibody (HA721462) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si LSD2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 92 kDa Observed band size: 92 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721462) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.