BCAR1 Recombinant Rabbit Monoclonal Antibody [JE36-56]
cat.: HA721465
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE36-56 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human BCAR1 aa 1-100 / 870. |
| Positive control: | HUVEC cell lysate, HeLa cell lysate, U-2 OS cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Cell junction, focal adhesion, Cytoplasm, Cell projection, axon. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P56945 Human | Q61140 Mouse | Q63767 Rat |
| Alternative names: | BCAR 1 Bcar1 BCAR1_HUMAN Breast cancer anti estrogen resistance 1 Breast cancer anti estrogen resistance 1 protein Breast cancer anti-estrogen resistance protein 1 CAS Cas scaffolding protein family member 1 CAS1 Cass1 Crk associated substrate Crk associated substrate p130Cas CRK-associated substrate CRKAS FLJ12176 FLJ45059 p130cas |
Images
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Fig1:
Western blot analysis of BCAR1 on different lysates with Rabbit anti-BCAR1 antibody (HA721465) at 1/1,000 dilution. Lane 1: HUVEC cell lysate Lane 2: HeLa cell lysate Lane 3: U-2 OS cell lysate Lane 4: HEK-293 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 93 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721465) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of BCAR1 on different lysates with Rabbit anti-BCAR1 antibody (HA721465) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-BCAR1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 93 kDa Observed band size: 110 kDa Exposure time: 94 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721465) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.