U2AF65 Recombinant Rabbit Monoclonal Antibody [JE36-33]
cat.: HA721466
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE36-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human U2AF65 aa 376-475 / 475. |
| Positive control: | HEK-293 cell lysate, HepG2 cell lysate, HeLa cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human liver tissue, mouse liver tissue, rat brain tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P26368 Human | P26369 Mouse Entrez Gene: 308335 Rat |
| Alternative names: | hU2AF(65) hU2AF65 Splicing factor U2AF 65 kDa subunit U2 (RNU2) small nuclear RNA auxiliary factor 2 U2 auxiliary factor 65 kDa subunit U2 small nuclear ribonucleoprotein auxiliary factor (65kD) U2 small nuclear RNA auxiliary factor 2 U2 snRNP auxiliary factor large subunit U2af2 U2AF2_HUMAN U2AF65 |
Images
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Fig1:
Western blot analysis of U2AF65 on different lysates with Rabbit anti-U2AF65 antibody (HA721466) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lane 5: SH-SY5Y cell lysate Lane 6: RAW264.7 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721466) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-U2AF65 antibody (HA721466) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721466) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-U2AF65 antibody (HA721466) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721466) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-U2AF65 antibody (HA721466) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721466) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.