Syntaxin 4 Recombinant Rabbit Monoclonal Antibody [JE35-92]
cat.: HA721467
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE35-92 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Syntaxin 4 aa 96-145 / 297. |
| Positive control: | HepG2 cell lysate, A549 cell lysate, Jurkat cell lysate, HeLa cell lysate, K-562 cell lysate, U-87 MG cell lysate, human lung tissue lysate, mouse skin tissue lysate, rat brain tissue lysate, human skin tissue, human prostate tissue. |
| Subcellular location: | Cell membrane, Cell projection, neuron projection. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q12846 Human | P70452 Mouse | Q08850 Rat |
| Alternative names: | NY REN 31 antigen p35 2 PLACENTAL Renal carcinoma antigen NY REN 31 Renal carcinoma antigen NY-REN-31 Stx4 STX4_HUMAN STX4A Syntaxin 4 syntaxin 4A (placental) Syntaxin 4A Syntaxin-4 |
Images
|
Fig1:
Western blot analysis of Syntaxin 4 on different lysates with Rabbit anti-Syntaxin 4 antibody (HA721467) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: K-562 cell lysate (20 µg/Lane) Lane 6: U-87 MG cell lysate (20 µg/Lane) Lane 7: Human lung tissue lysate (40 µg/Lane) Lane 8: Mouse skin tissue lysate (40 µg/Lane) Lane 9: rat brain tissue lysate (40 µg/Lane) Predicted band size: 34 kDa Observed band size: 34/30 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721467) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Syntaxin 4 antibody (HA721467) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721467) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-Syntaxin 4 antibody (HA721467) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721467) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.