Cystathionase Recombinant Rabbit Monoclonal Antibody [JE35-91]
cat.: HA721468
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JE35-91 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Cystathionase aa 1-405 / 405. |
| Positive control: | HepG2 cell lysate, 293T cell lysate, K-562 cell lysate, HEK-293 cell lysate, NCI-H441 cell lysate, A431 cell lysate, F9 cell lysate, human liver tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, human kidney tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IP |
1:1,000-1:10,000 1-2μg/sample |
| Uniprot #: | SwissProt: P32929 Human | Q8VCN5 Mouse | P18757 Rat |
| Alternative names: | CGL_HUMAN CTH cystathionase (cystathionine gamma-lyase) Cystathionine gamma lyase Cystathionine gamma-lyase Cysteine desulfhydrase Gamma cystathionase Gamma-cystathionase Homoserine deaminase Homoserine dehydratase MGC9471 |
Images
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Fig1:
Western blot analysis of Cystathionase on different lysates with Rabbit anti-Cystathionase antibody (HA721468) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: HEK-293 cell lysate (20 µg/Lane) Lane 5: NCI-H441 cell lysate (20 µg/Lane) Lane 6: A431 cell lysate (20 µg/Lane) Lane 7: F9 cell lysate (20 µg/Lane) Lane 8: Human liver tissue lysate (40 µg/Lane) Lane 9: Mouse liver tissue lysate (40 µg/Lane) Lane 10: Rat liver tissue lysate (40 µg/Lane) Lane 11: Human kidney tissue lysate (40 µg/Lane) Lane 12: Mouse kidney tissue lysate (40 µg/Lane) Lane 13: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721468) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Cystathionase on different lysates with Rabbit anti-Cystathionase antibody (HA721468) at 1/10,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Cystathionase KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721468) at 1/10,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Cystathionase was immunoprecipitated in 0.2mg A431 cell lysate with HA721468 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA721468 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input) Lane 2: HA721468 IP in A431 cell lysate Lane 3: Rabbit IgG instead of HA721468 in A431 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.