StAR Recombinant Rabbit Monoclonal Antibody [JE32-23]
cat.: HA721469
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE32-23 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human StAR aa 235-285. |
| Positive control: | K-562 cell lysates, human adrenal gland tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P49675 Human |
| Alternative names: | Cholesterol trafficker Luteinizing hormone induced protein mitochondrial Mitochondrial steroid acute regulatory protein StAR StAR related lipid transfer (START) domain containing 1 STAR_HUMAN StARD1 START domain containing 1 START domain containing protein 1 START domain-containing protein 1 Steroid acute regulatory protein Steroidogenic acute regulator Steroidogenic acute regulatory protein Steroidogenic acute regulatory protein mitochondrial |
Images
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Fig1:
Western blot analysis of StAR on K-562 cell lysates with Rabbit anti-StAR antibody (HA721469) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 32 kDa Observed band size: 26 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721469) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue with Rabbit anti-StAR antibody (HA721469) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721469) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.