Phospho-Chk1 (S280) Recombinant Rabbit Monoclonal Antibody [JE47-66]
cat.: HA721471
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE47-66 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Ser280 of human Chk1. |
| Positive control: | 293T treated with 100nM Calyculin A for 15 minutes cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate. |
| Subcellular location: | Nucleus, Chromosome, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O14757 Human | O35280 Mouse | Q91ZN7 Rat |
| Alternative names: | C85740 Cell cycle checkpoint kinase Checkpoint , S. pombe, homolog of, 1 Checkpoint kinase 1 Checkpoint kinase 1 homolog (S. pombe) CHEK 1 Chek1 Chk 1 Chk1 CHK1 checkpoint homolog (S. pombe) CHK1_HUMAN EC 2.7.11.1 rad27 Serine/threonine protein kinase Chk1 Serine/threonine-protein kinase CHK1 STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) |
Images
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Fig1:
Western blot analysis of Phospho-Chk1 (S280) on different lysates with Rabbit anti-Phospho-Chk1 (S280) antibody (HA721471) at 1/1,000 dilution. Lane 1: 293T whole cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: 293T treated with 100nM Calyculin A for 15 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721471) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Phospho-Chk1 (S280) on different lysates with Rabbit anti-Phospho-Chk1 (S280) antibody (HA721471) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721471) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Western blot analysis of Phospho-Chk1 (S280) on different lysates with Rabbit anti-Phospho-Chk1 (S280) antibody (HA721471) at 1/1,000 dilution. Lane 1: PC-12 whole cell lysate Lane 2: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721471) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.