Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Mouse, Rat |
Applications: | WB, IHC-P, IF-Tissue |
Clonality: | Monoclonal |
Clone number: | PSH0-71 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 29 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within mouse Ms4a10 aa 218-267 / 267. |
Positive control: | Mouse small intestine tissue, rat small intestine tissue. |
Subcellular location: | Membrane. |
Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:100-1:500 1:200 |
Uniprot #: | SwissProt: Q99N03 Mouse | D4A504 Rat |
Alternative names: | Membrane-spanning 4-domains subfamily A member 10 Ms4a10 |
Fig1:
Western blot analysis of Ms4a10 on different lysates with Rabbit anti-Ms4a10 antibody (HA721472) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa overexpress mouse Ms4a10 whole cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 29 kDa Observed band size: 29/25 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721472) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Ms4a10 antibody (HA721472) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721472) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-Ms4a10 antibody (HA721472) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721472) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Fig4:
Immunofluorescence analysis of paraffin-embedded mouse small intestine tissue labeling Ms4a10 with Rabbit anti-Ms4a10 antibody (HA721472) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721472, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig5:
Immunofluorescence analysis of paraffin-embedded rat small intestine tissue labeling Ms4a10 with Rabbit anti-Ms4a10 antibody (HA721472) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721472, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |