Syndecan 4 Recombinant Rabbit Monoclonal Antibody [PSH0-72]
cat.: HA721473
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH0-72 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Syndecan 4 Extracellular. |
| Positive control: | HepG2 cell lysate, Jurkat cell lysate, HeLa cell lysate, U-87 MG cell lysate, A549 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate. |
| Subcellular location: | Membrane, Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P31431 Human |
| Alternative names: | Amphiglycan MGC22217 OTTHUMP00000031788 ryudocan amphiglycan Ryudocan Ryudocan core protein SDC 4 Sdc4 SDC4_HUMAN SYND 4 SYND4 syndecan 4 syndecan 4 (amphiglycan, ryudocan) syndecan proteoglycan 4 Syndecan-4 Syndecan4 |
Images
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Fig1:
Western blot analysis of Syndecan 4 on different lysates with Rabbit anti-Syndecan 4 antibody (HA721473) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: U-87 MG cell lysate Lane 5: A549 cell lysate Lane 6: MCF7 cell lysate Lane 7: MDA-MB-231 cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721473) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.