DcR1 Recombinant Rabbit Monoclonal Antibody [JE58-51]
cat.: HA721475
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE58-51 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human DcR1 aa 200-300. |
| Positive control: | JAR cell lysate, Jurkat cell lysate, A549 cell lysate, HeLa cell lysate, Raji cell lysate, Jurkat. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:100 1:500-1:1,000 |
| Uniprot #: | SwissProt: O14798 Human |
| Alternative names: | Antagonist decoy receptor for TRAIL/Apo 2L Antagonist decoy receptor for TRAIL/Apo-2L CD263 Cytotoxic TRAIL receptor 3 DcR1 DCR1 TNFR Decoy receptor 1 Decoy TRAIL receptor without death domain LIT Lymphocyte inhibitor of TRAIL MGC149501 MGC149502 TNF related apoptosis inducing ligand receptor 3 TNF-re ated apoptosis inducing ligand receptor 3 TNF-related apoptosis-inducing ligand receptor 3 TNFRSF10C TR10C_HUMAN TRAIL R3 TRAIL receptor 3 Trail receptor without an intracellular domain TRAIL-R3 TRAILR3 TRID Tumor necrosis factor receptor superfamily member 10C Tumor necrosis factor receptor superfamily, member 10c, decoy without an intracellular domain |
Images
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Fig1:
Western blot analysis of DcR1 on different lysates with Rabbit anti-DcR1 antibody (HA721475) at 1/1,000 dilution. Lane 1: JAR cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lane 4: HeLa cell lysate Lane 5: Raji cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 27 kDa Observed band size: 27/70/80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721475) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Jurkat cells labeling DcR1 with Rabbit anti-DcR1 antibody (HA721475) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-DcR1 antibody (HA721475) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of Jurkat cells labeling DcR1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721475, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.