DDR2 Recombinant Rabbit Monoclonal Antibody [JE31-45]
cat.: HA721479
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE31-45 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 97 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human DDR2 aa 806-855 / 855. |
| Positive control: | HeLa cell lysate, SH-SY5Y cell lysate, L929 cell lysate, NIH/3T3 cell lysate, U-2 OS cell lysate, A375 cell lysate, PC-12 cell lysate, rat skeletal muscle tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q16832 Human | Q62371 Mouse Entrez Gene: 685781 Rat |
| Alternative names: | CD167 antigen-like family member B CD167b CD167b antigen Cell migration inducing protein 20 DDR 2 DDR2 DDR2_HUMAN Discoidin domain containing receptor 2 Discoidin domain receptor 2 Discoidin domain receptor family member 2 discoidin domain receptor tyrosine kinase 2 Discoidin domain-containing receptor 2 discoidin domain-containing receptor tyrosine kinase 2 Hydroxyaryl protein kinase MIG20a Migration inducing gene 16 protein Neurotrophic tyrosine kinase Neurotrophic tyrosine kinase receptor related 3 NTRKR 3 NTRKR3 Receptor protein tyrosine kinase TKT Receptor protein-tyrosine kinase TKT Receptor related 3 receptor-related 3 TKT TYRO 10 TYRO10 Tyrosine kinase receptor related to neurotrophic TRK Tyrosine protein kinase TYRO 10 Tyrosine protein kinase TYRO10 Tyrosine-protein kinase TYRO10 Tyrosylprotein kinase |
Images
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Fig1:
Western blot analysis of DDR2 on different lysates with Rabbit anti-DDR2 antibody (HA721479) at 1/1,000 dilution. Lane 1: HeLa cell lysate (40 µg/Lane) Lane 2: SH-SY5Y cell lysate (40 µg/Lane) Lane 3: L929 cell lysate (40 µg/Lane) Lane 4: NIH/3T3 cell lysate (40 µg/Lane) Lane 5: U-2 OS cell lysate (15 µg/Lane) Lane 6: A375 cell lysate (15 µg/Lane) Lane 7: PC-12 cell lysate (10 µg/Lane) Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721479) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-DDR2 antibody (HA721479) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721479) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.