DDR2 Recombinant Rabbit Monoclonal Antibody [JE31-45]
cat.: HA721479
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE31-45
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 97 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human DDR2 aa 806-855 / 855.
Positive control: HeLa cell lysate, SH-SY5Y cell lysate, L929 cell lysate, NIH/3T3 cell lysate, U-2 OS cell lysate, A375 cell lysate, PC-12 cell lysate, rat skeletal muscle tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:200
Uniprot #: SwissProt: Q16832 Human | Q62371 Mouse
Entrez Gene: 685781 Rat
Alternative names: CD167 antigen-like family member B CD167b CD167b antigen Cell migration inducing protein 20 DDR 2 DDR2 DDR2_HUMAN Discoidin domain containing receptor 2 Discoidin domain receptor 2 Discoidin domain receptor family member 2 discoidin domain receptor tyrosine kinase 2 Discoidin domain-containing receptor 2 discoidin domain-containing receptor tyrosine kinase 2 Hydroxyaryl protein kinase MIG20a Migration inducing gene 16 protein Neurotrophic tyrosine kinase Neurotrophic tyrosine kinase receptor related 3 NTRKR 3 NTRKR3 Receptor protein tyrosine kinase TKT Receptor protein-tyrosine kinase TKT Receptor related 3 receptor-related 3 TKT TYRO 10 TYRO10 Tyrosine kinase receptor related to neurotrophic TRK Tyrosine protein kinase TYRO 10 Tyrosine protein kinase TYRO10 Tyrosine-protein kinase TYRO10 Tyrosylprotein kinase
Images
HA721479_1.jpg Fig1: Western blot analysis of DDR2 on different lysates with Rabbit anti-DDR2 antibody (HA721479) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (40 µg/Lane)
Lane 2: SH-SY5Y cell lysate (40 µg/Lane)
Lane 3: L929 cell lysate (40 µg/Lane)
Lane 4: NIH/3T3 cell lysate (40 µg/Lane)
Lane 5: U-2 OS cell lysate (15 µg/Lane)
Lane 6: A375 cell lysate (15 µg/Lane)
Lane 7: PC-12 cell lysate (10 µg/Lane)

Predicted band size: 97 kDa
Observed band size: 97 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721479) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721479_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-DDR2 antibody (HA721479) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721479) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.