Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Mouse, Rat, Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE33-89 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 52 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human Steroidogenic Factor 1 aa 312-461 / 461. |
Positive control: | Mouse spleen tissue lysate, rat ovary tissue lysate. |
Subcellular location: | Nucleus. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: Q13285 Human | P33242 Mouse | P50569 Rat |
Alternative names: | AD4BP Adrenal 4 binding protein Adrenal 4-binding protein ELP FTZ 1 FTZ1 FTZF 1 FTZF1 Fushi tarazu factor (Drosophila) homolog 1 Fushi tarazu factor homolog 1 hSF 1 NR5A1 Nuclear receptor AdBP4 Nuclear receptor subfamily 5 group A member 1 POF7 SF 1 SF-1 SF1 SPGF8 SRXY3 Steroid hormone receptor Ad4BP Steroidogenic factor 1 Steroidogenic factor 1 nuclear receptor STF 1 STF-1 STF1 STF1_HUMAN |
Fig1:
Western blot analysis of Steroidogenic Factor 1 on different lysates with Rabbit anti-Steroidogenic Factor 1 antibody (HA721482) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat ovary tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 50 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721482) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |