Steroidogenic Factor 1 Recombinant Rabbit Monoclonal Antibody [JE33-89]
cat.: HA721482
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Rat, Human
Applications: WB
Clonality: Monoclonal
Clone number: JE33-89
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 52 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Steroidogenic Factor 1 aa 312-461 / 461.
Positive control: Mouse spleen tissue lysate, rat ovary tissue lysate.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q13285 Human | P33242 Mouse | P50569 Rat
Alternative names: AD4BP Adrenal 4 binding protein Adrenal 4-binding protein ELP FTZ 1 FTZ1 FTZF 1 FTZF1 Fushi tarazu factor (Drosophila) homolog 1 Fushi tarazu factor homolog 1 hSF 1 NR5A1 Nuclear receptor AdBP4 Nuclear receptor subfamily 5 group A member 1 POF7 SF 1 SF-1 SF1 SPGF8 SRXY3 Steroid hormone receptor Ad4BP Steroidogenic factor 1 Steroidogenic factor 1 nuclear receptor STF 1 STF-1 STF1 STF1_HUMAN
Images
HA721482_1.jpg Fig1: Western blot analysis of Steroidogenic Factor 1 on different lysates with Rabbit anti-Steroidogenic Factor 1 antibody (HA721482) at 1/1,000 dilution.

Lane 1: Mouse spleen tissue lysate
Lane 2: Rat ovary tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 52 kDa
Observed band size: 50 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721482) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.