CRALBP Recombinant Rabbit Monoclonal Antibody [PSH0-74]
cat.: HA721483
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PSH0-74 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36.5 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CRALBP aa 1-317 / 317. |
| Positive control: | SK-MEL-28 cell lysate, mouse eyeball tissue lysate, rat eyeball tissue lysate, mouse eyeball tissue, mouse retina tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:200-1:500 1:200 |
| Uniprot #: | SwissProt: P12271 Human | Q9Z275 Mouse Entrez Gene: 293049 Rat |
| Alternative names: | Cellular retinaldehyde binding protein 1 Cellular retinaldehyde binding protein Cellular retinaldehyde-binding protein MGC3663 Retinaldehyde binding protein 1 Retinaldehyde-binding protein 1 RLBP 1 RLBP1 RLBP1_HUMAN |
Images
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Fig1:
Western blot analysis of CRALBP on different lysates with Rabbit anti-CRALBP antibody (HA721483) at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: HepG2 cell lysate (negative cell) Lane 3: Mouse eyeball tissue lysate Lane 4: Rat eyeball tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 36.5 kDa Observed band size: 30 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721483) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue with Rabbit anti-CRALBP antibody (HA721483) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721483) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-CRALBP antibody (HA721483) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721483) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunofluorescence analysis of paraffin-embedded mouse eyeball tissue labeling CRALBP with Rabbit anti-CRALBP antibody (HA721483) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721483, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.