Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE40-73 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 22 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within C terminal Human Glutathione Peroxidase 2/GPX2. |
Positive control: | SW620 cell lysate, LoVo cell lysate, HT-29 cell lysate, AGS cell lysate. |
Subcellular location: | Cytoplasm, cytosol. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: P18283 Human |
Alternative names: | Gastrointestinal glutathione peroxidase GI GPx Glutathione peroxidase 2 (gastrointestinal) Glutathione peroxidase 2 Glutathione peroxidase gastrointestinal Glutathione peroxidase related protein 2 Glutathione peroxidase-gastrointestinal Glutathione peroxidase-related protein 2 GPRP GPRP-2 GPx 2 GPx-2 GPx-GI GPX2 GPX2_HUMAN GSHPx 2 GSHPx GI GSHPx-2 GSHPx-GI |
Fig1:
Western blot analysis of Glutathione Peroxidase 2 on different lysates with Rabbit anti-Glutathione Peroxidase 2 antibody (HA721485) at 1/1,000 dilution. Lane 1: SW620 cell lysate Lane 2: LoVo cell lysate Lane 3: HT-29 cell lysate Lane 4: AGS cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721485) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |