Glutathione Peroxidase 2 Recombinant Rabbit Monoclonal Antibody [JE40-73]
cat.: HA721485
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE40-73
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 22 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C terminal Human Glutathione Peroxidase 2/GPX2.
Positive control: SW620 cell lysate, LoVo cell lysate, HT-29 cell lysate, AGS cell lysate.
Subcellular location: Cytoplasm, cytosol.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P18283 Human
Alternative names: Gastrointestinal glutathione peroxidase GI GPx Glutathione peroxidase 2 (gastrointestinal) Glutathione peroxidase 2 Glutathione peroxidase gastrointestinal Glutathione peroxidase related protein 2 Glutathione peroxidase-gastrointestinal Glutathione peroxidase-related protein 2 GPRP GPRP-2 GPx 2 GPx-2 GPx-GI GPX2 GPX2_HUMAN GSHPx 2 GSHPx GI GSHPx-2 GSHPx-GI
Images
HA721485_1.jpg Fig1: Western blot analysis of Glutathione Peroxidase 2 on different lysates with Rabbit anti-Glutathione Peroxidase 2 antibody (HA721485) at 1/1,000 dilution.

Lane 1: SW620 cell lysate
Lane 2: LoVo cell lysate
Lane 3: HT-29 cell lysate
Lane 4: AGS cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 22 kDa
Observed band size: 22 kDa

Exposure time: 1 minute 17 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721485) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.