Pumilio 2 Recombinant Rabbit Monoclonal Antibody [JE37-76]
cat.: HA721486
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE37-76 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 114 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Pumilio 2 aa 1-100. |
| Positive control: | A549 cell lysate, U-2 OS cell lysate, MCF7 cell lysate, U-2 OS. |
| Subcellular location: | Cytoplasm, Cytoplasmic granule, perinuclear region. |
| Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q8TB72 Human |
| Alternative names: | FLJ36528 KIAA0235 MGC138251 MGC138253 PUM 2 PUM2 PUM2_HUMAN PUMH 2 PUMH2 Pumilio (Drosphila) homolog 2 Pumilio homolog 2 (Drosophila) Pumilio homolog 2 Pumilio-2 Pumilio2 PUML2 |
Images
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Fig1:
Western blot analysis of Pumilio 2 on different lysates with Rabbit anti-Pumilio 2 antibody (HA721486) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 114 kDa Observed band size: 140 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721486) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of U-2 OS cells labeling Pumilio 2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721486, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.