Bmf Recombinant Rabbit Monoclonal Antibody [JE33-57]
cat.: HA721487
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE33-57 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Bmf aa 1-184 / 184. |
| Positive control: | Mouse liver tissue lysate, rat liver tissue lysate, mouse spleen tissue lysate, rat spleen tissue lysate, mouse pancreas tissue lysate, rat pancreas tissue lysate, mouse lung tissue lysate, rat ovary tissue lysate. |
| Subcellular location: | Acrosomal vesicle, cytosol, mitochondrial outer membrane, myosin complex, plasma membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q96LC9 Human | Q91ZE9 Mouse | Q8K589 Rat |
| Alternative names: | Bcl 2 modifying factor Bcl-2-modifying factor Bcl2 modifying factor Bmf BMF_HUMAN FLJ00065 |
Images
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Fig1:
Western blot analysis of Bmf on different lysates with Rabbit anti-Bmf antibody (HA721487) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lane 3: Mouse spleen tissue lysate Lane 4: Rat spleen tissue lysate Lane 5: Mouse pancreas tissue lysate Lane 6: Rat pancreas tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 21 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721487) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Bmf on different lysates with Rabbit anti-Bmf antibody (HA721487) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate Lane 2: Rat liver tissue lysate Lane 3: Rat ovary tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 21 kDa Observed band size: 30 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721487) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.