Muscarinic Acetylcholine Receptor 2 Recombinant Rabbit Monoclonal Antibody [JE38-35]
cat.: HA721490
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE38-35 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Muscarinic Acetylcholine Receptor 2 aa 140-184 / 466. |
| Positive control: | HepG2 cell lysate, SH-SY5Y cell lysate, U-87 MG cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, human colon carcinoma tissue, mouse kidney tissue, SH-SY5Y. |
| Subcellular location: | Cell membrane, Postsynaptic cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:1,000 1:1,000 1:100 1:500-1:1,000 |
| Uniprot #: | SwissProt: P08172 Human | Q9ERZ4 Mouse | P10980 Rat |
| Alternative names: | 7TM receptor Acetylcholine receptor, muscarinic, 2 AChR Acm2 ACM2_HUMAN Cholinergic receptor muscarinic 2 Cholinergic receptor, muscarinic 2, cardiac Cholinergic receptor, muscarinic 2, isoform a Cholinergic receptor, muscarinic 2a CHRM 2 CHRM2 chrm2a CM2 FLJ43243 HM 2 HM2 M2 M2 muscarinic receptor M2-mAChR MGC120006 MGC120007 Muscarinic acetylcholine receptor M2 Muscarinic M2 receptor |
Images
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Fig1:
Western blot analysis of Muscarinic Acetylcholine Receptor 2 on different lysates with Rabbit anti-Muscarinic Acetylcholine Receptor 2 antibody (HA721490) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: U-87 MG cell lysate Lane 4: Neuro-2a cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721490) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Muscarinic Acetylcholine Receptor 2 antibody (HA721490) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721490) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Muscarinic Acetylcholine Receptor 2 antibody (HA721490) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721490) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of SH-SY5Y cells labeling Muscarinic Acetylcholine Receptor 2 with Rabbit anti-Muscarinic Acetylcholine Receptor 2 antibody (HA721490) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Muscarinic Acetylcholine Receptor 2 antibody (HA721490) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃.Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Flow cytometric analysis of SH-SY5Y cells labeling Muscarinic Acetylcholine Receptor 2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721490, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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