Rad50 Recombinant Rabbit Monoclonal Antibody [JE60-04]
cat.: HA721492
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE60-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 154 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Rad50 aa 1,162-1,312 / 1,312. |
| Positive control: | HeLa cell lysate, K-562 cell lysate, U-87 MG cell lysate, U-937 cell lysate, Caco-2 cell lysate, mouse testis tissue lysate, rat testis tissue lysate. |
| Subcellular location: | Nucleus, Chromosome, telomere. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q92878 Human | P70388 Mouse | Q9JIL8 Rat |
| Alternative names: | DNA repair protein RAD50 hRAD50 hsRAD50 Mrell NBSLD Rad 50 RAD50 2 rad50 RAD50 homolog (S cerevisiae) RAD50 homolog RAD50 PEN RAD50, S. cerevisiae, homolog of RAD50_HUMAN RAD502 Rad50l Truncated RAD50 protein |
Images
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Fig1:
Western blot analysis of Rad50 on different lysates with Rabbit anti-Rad50 antibody (HA721492) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: U-87 MG cell lysate (20 µg/Lane) Lane 4: U-937 cell lysate (20 µg/Lane) Lane 5: Caco-2 cell lysate (20 µg/Lane) Lane 6: Mouse testis tissue lysate (40 µg/Lane) Lane 7: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 154 kDa Observed band size: 154 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721492) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.