DDX17 Recombinant Rabbit Monoclonal Antibody [JE35-01]
cat.: HA721493
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Monoclonal
Clone number: JE35-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 80 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human DDX17 aa 100-200.
Positive control: HEK-293 cell lysate, HeLa cell lysate, K-562 cell lysate, MCF7 cell lysate, SK-OV-3 cell lysate, human breast carcinoma tissue, human colon carcinoma tissue, rat ovary tissue, MCF7.
Subcellular location: Nucleus, nucleolus, Cytoplasm, cytosol.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:1,000
1:2,000
1:100
1:500-1:1,000
Uniprot #: SwissProt: Q92841 Human
Entrez Gene: 315133 Rat
Alternative names: Ddx17 DDX17_HUMAN DEAD (Asp Glu Ala Asp) box helicase 17 DEAD (Asp Glu Ala Asp) box polypeptide 17 DEAD box helicase 17 DEAD box protein 17 DEAD box protein p72 DEAD/H (Asp Glu Ala Asp/His) box polypeptide 17 P72 Probable ATP-dependent RNA helicase DDX17 RH70 RNA dependent helicase p72 RNA-dependent helicase p72
Images
HA721493_1.jpg Fig1: Western blot analysis of DDX17 on different lysates with Rabbit anti-DDX17 antibody (HA721493) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: SK-OV-3 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 80 kDa
Observed band size: 70/75 kDa

Exposure time: 1 minute 21 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721493) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721493_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-DDX17 antibody (HA721493) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721493) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721493_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-DDX17 antibody (HA721493) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721493) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721493_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-DDX17 antibody (HA721493) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721493) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721493_5.jpg Fig5: Immunocytochemistry analysis of MCF7 cells labeling DDX17 with Rabbit anti-DDX17 antibody (HA721493) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DDX17 antibody (HA721493) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721493_6.jpg Fig6: Flow cytometric analysis of MCF7 cells labeling DDX17.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721493, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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