CYP1A2 Recombinant Rabbit Monoclonal Antibody [JE39-22]
cat.: HA721495
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE39-22 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CYP1A2 aa 200-300. |
| Positive control: | HepG2 cell lysates, HepG2. |
| Subcellular location: | Endoplasmic reticulum membrane, Microsome membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:250 1:500-1:1,000 |
| Uniprot #: | SwissProt: P05177 Human |
| Alternative names: | Aryl hydrocarbon hydroxylase CP 12 CP12 CP1A2_HUMAN CYP1A2 CYPIA2 Cytochrome P(3)450 Cytochrome P450 1A2 Cytochrome P450 4 Cytochrome P450 family 1 polypeptide 2 Cytochrome P450 family 1 subfamily A polypeptide 2 Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2 Cytochrome P450-P3 Cytochrome P4501A2 Dioxin inducable P3 450 Flavoprotein linked monooxygenase Flavoprotein-linked monooxygenase Microsomal monooxygenase P(3)450 P3 450 P450 4 P450 form 4 P450 P3 P450(PA) Xenobiotic monooxygenase |
Images
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Fig1:
Western blot analysis of CYP1A2 on HepG2 cell lysates with Rabbit anti-CYP1A2 antibody (HA721495) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 58 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721495) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling CYP1A2 with Rabbit anti-CYP1A2 antibody (HA721495) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CYP1A2 antibody (HA721495) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HepG2 cells labeling CYP1A2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721495, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Western blot analysis of CYP1A2 on different lysates with Rabbit anti-CYP1A2 antibody (HA721495) at 1/1,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si CYP1A2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 58 kDa Observed band size: 50 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721495) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.