GABA Transporter 1 / GAT 1 Recombinant Rabbit Monoclonal Antibody [JE34-61]
cat.: HA721496
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IHC-Fr, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE34-61 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GABA Transporter 1/ GAT 1 aa 1-52. |
| Positive control: | Mouse cerebellum tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human brain tissue, human liver tissue, mouse cerebellum tissue, mouse retina tissue, rat brain tissue, rat retina tissue. |
| Subcellular location: | Cell membrane, Presynapse. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IF-Tissue |
1:1,000 1:500-1:2,000 1:1,000 1:500 |
| Uniprot #: | SwissProt: P30531 Human | P31648 Mouse | P23978 Rat |
| Alternative names: | GABATHG GABATR GABT 1 GABT1 GAT-1 GAT1 SC6A1_HUMAN Slc6a1 Sodium and chloride dependent GABA transporter 1 Sodium- and chloride-dependent GABA transporter 1 Solute carrier family 6 (neurotransmitter transporter GABA) member 1 Solute carrier family 6 member 1 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required |
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Fig2:
Western blot analysis of GABA Transporter 1 / GAT 1 on different lysates with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/1,000 dilution. Lane 1: Mouse cerebellum tissue lysate Lane 2: Mouse cerebellum tissue lysate (70℃ heat) Lane 3: Mouse brain tissue lysate Lane 4: Mouse brain tissue lysate (70℃ heat) Lane 5: Rat brain tissue lysate Lane 6: Rat brain tissue lysate (70℃ heat) Lysates/proteins at 30 µg/Lane. Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: Lane 1-4: 1 minute 40 seconds; Lane 5-6: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721496) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded rat retina tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig10:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GABA Transporter 1 / GAT 1 antibody (HA721496) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721496) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig11:
Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1:500 |
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Fig12:
Application: IF-tissue Species: Rat Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1:500 |
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Fig13:
Application: IF-tissue Species: Human Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1:500 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.