| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE40-44 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 122 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SNF2H aa 1,003-1,052 / 1,052. |
| Positive control: | HeLa cell lysate, U-2 OS cell lysate, Jurkat cell lysate, HepG2 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O60264 Human | Q91ZW3 Mouse Entrez Gene: 307766 Rat |
| Alternative names: | 4933427E24Rik D030040M08Rik D330027N15Rik EC 3.6.1.- hISWI hSNF2H ISWI MommeD4 SMARCA5 SMCA5_HUMAN Snf2h Sucrose nonfermenting like 5 Sucrose nonfermenting protein 2 homolog Sucrose nonfermenting, yeast, homolog of SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 WCRF135 |
|
Fig1:
Western blot analysis of SNF2H on different lysates with Rabbit anti-SNF2H antibody (HA721500) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-2 OS cell lysate Lane 3: Jurkat cell lysate Lane 4: HepG2 cell lysate Lane 5: A549 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 122 kDa Observed band size: 122 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721500) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |