TRPM7 Recombinant Rabbit Monoclonal Antibody [JE38-36]
cat.: HA721501
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE38-36 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 213 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TRPM7 aa 1,816-1,865 / 1,865. |
| Positive control: | HeLa cell lysate, K-562 cell lysate, HepG2 cell lysate, HCT 116 cell lysate, HeLa. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q96QT4 Human |
| Alternative names: | ALSPDC CHAK CHAK1 Channel kinase 1 Channel-kinase 1 Long transient receptor potential channel 7 LTrpC-7 LTrpC7 Transient receptor potential cation channel subfamily M member 7 TRP PLIK TRPM7 TRPM7_HUMAN |
Images
|
Fig1:
Western blot analysis of TRPM7 on different lysates with Rabbit anti-TRPM7 antibody (HA721501) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: HepG2 cell lysate Lane 4: HCT 116 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 213 kDa Observed band size: 250 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721501) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HeLa cells labeling TRPM7. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA721501, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.