SAP97 Recombinant Rabbit Monoclonal Antibody [JE39-74]
cat.: HA721502
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE39-74 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 100 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N terminal Human SAP97. |
| Positive control: | MCF7 cell lysate, HepG2 cell lysate, U-87 MG cell lysate, SiHa cell lysate, NCI-H226 cell lysate, NIH/3T3 cell lysate, mouse liver tissue, rat cerebellum tissue, SiHa. |
| Subcellular location: | Membrane, Basolateral cell membrane, Endoplasmic reticulum membrane, Postsynaptic density, Synapse, Cell membrane, sarcolemma, Apical cell membrane, Cell junction, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:200 1:500-1:1,000 |
| Uniprot #: | SwissProt: Q12959 Human | Q811D0 Mouse | Q62696 Rat |
| Alternative names: | Discs large homolog 1 discs large, Drosophila, homolog of, 1 discs, large homolog 1 (Drosophila) Disks large homolog 1 dJ1061C18.1.1 DKFZp761P0818 DKFZp781B0426 DLG 1 DLG1 DLG1_HUMAN DLGH 1 DLGH1 hDlg Presynaptic protein SAP97 SAP 97 SAP-97 SAP97 Synapse associated protein 97 Synapse-associated protein 97 |
Images
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Fig1:
Western blot analysis of SAP97 on different lysates with Rabbit anti-SAP97 antibody (HA721502) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: U-87 MG cell lysate Lane 4: SiHa cell lysate Lane 5: NCI-H226 cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721502) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of SAP97 on different lysates with Rabbit anti-SAP97 antibody (HA721502) at 1/2,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD SAP97 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 100 kDa Observed band size: 120 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721502) at 1/2,000 dilution was used in 2% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-SAP97 antibody (HA721502) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721502) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-SAP97 antibody (HA721502) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721502) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of SiHa cells labeling SAP97. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721502, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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