TRF1 Recombinant Rabbit Monoclonal Antibody [JE59-70]
cat.: HA721505
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE59-70 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TRF1 aa 51-150 / 439. |
| Positive control: | Jurkat cell lysate, K-562 cell lysate, U-2 OS cell lysate, MCF7 cell lysate, RAW264.7 cell lysate. |
| Subcellular location: | Nucleus, Cytoplasm, cytoskeleton, spindle, Chromosome, telomere. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P54274 Human | P70371 Mouse |
| Alternative names: | hTRF1 AS NIMA interacting protein 2 NIMA-interacting protein 2 PIN 2 PIN2 t TRF1 Telomeric protein Pin2 Telomeric protein Pin2/TRF1 Telomeric repeat binding factor (NIMA interacting) 1 Telomeric repeat binding factor 1 Telomeric repeat binding protein 1 Telomeric repeat-binding factor 1 TERF 1 Terf1 TERF1_HUMAN TRBF 1 TRBF1 TRF 1 TRF TTAGGG repeat binding factor 1 TTAGGG repeat-binding factor 1 |
Images
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Fig1:
Western blot analysis of TRF1 on different lysates with Rabbit anti-TRF1 antibody (HA721505) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: U-2 OS cell lysate Lane 4: MCF7 cell lysate Lane 5: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721505) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.