TRIM25 Recombinant Rabbit Monoclonal Antibody [JE57-43]
cat.: HA721506
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE57-43 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human TRIM25/EFP aa 100-200. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, K-562 cell lysate, A549 cell lysate, MCF7 cell lysate, Jurkat cell lysate, 293T cell lysate, HeLa. |
| Subcellular location: | Cytoplasm, Stress granule, Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q14258 Human |
| Alternative names: | E3 ubiquitin/ISG15 ligase TRIM25 EFP Estrogen responsive finger protein Estrogen-responsive finger protein RING finger protein 147 RNF 147 RNF147 TRI25 TRI25_HUMAN TRIM 25 Trim25 Tripartite motif containing 25 Tripartite motif containing protein 25 Tripartite motif protein TRIM25 Tripartite motif-containing protein 25 Tripartite motifcontaining25 Ubiquitin/ISG15-conjugating enzyme TRIM25 Z147 Zinc finger protein 147 (estrogen responsive finger protein) Zinc finger protein 147 Zinc finger protein-147 ZNF 147 ZNF147 |
Images
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Fig1:
Western blot analysis of TRIM25 on different lysates with Rabbit anti-TRIM25 antibody (HA721506) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: A549 cell lysate Lane 5: MCF7 cell lysate Lane 6: Jurkat cell lysate Lane 7: 293T cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721506) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of TRIM25 on different lysates with Rabbit anti-TRIM25 antibody (HA721506) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si TRIM25 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 71 kDa Observed band size: 71 kDa Exposure time: 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721506) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of HeLa cells labeling TRIM25 with Rabbit anti-TRIM25 antibody (HA721506) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TRIM25 antibody (HA721506) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.