CEACAM1 Recombinant Rabbit Monoclonal Antibody [PSH0-78]
cat.: HA721507
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH0-78 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CEACAM1 aa 35-428 / 526. |
| Positive control: | SW480 cell lysate, A549 cell lysate, human kidney tissue, human liver tissue. |
| Subcellular location: | Cell membrane, Lateral cell membrane, Apical cell membrane, Basal cell membrane, Cell junction, adherens junction. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P13688 Human |
| Alternative names: | Antigen CD66 BGP 1 BGP BGP-1 BGPI Biliary glycoprotein 1 Biliary glycoprotein adhesion molecule Biliary glycoprotein Carcinoembryonic antigen related cell adhesion molecule 1 carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) Carcinoembryonic antigen-related cell adhesion molecule 1 CD66a CD66a antigen CEACAM1 CEAM1_HUMAN meconium antigen 100 |
Images
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Fig1:
Western blot analysis of CEACAM1 on different lysates with Rabbit anti-CEACAM1 antibody (HA721507) at 1/1,000 dilution and Mouse anti-6X His-tag antibody (HA600030) at 1/1,000 dilution. Lane 1: His-tagged human CEACAM-1 recombinant protein aa (aa35-428) 20ng Lane 2: His-tagged human CEACAM-5 recombinant protein aa (aa35-422) 20ng Lane 3: His-tagged human CEACAM-6 recombinant protein aa (aa35-326) 20ng Lane 4: His-tagged human CEACAM-8 recombinant protein aa (aa35-335) 20ng Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721507) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CEACAM1 on different lysates with Rabbit anti-CEACAM1 antibody (HA721507) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: SW480 cell lysate (no heat) Lane 3: A549 cell lysate Lane 4: HeLa cell lysate (no heat) (negative) Lane 5: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 58 kDa Observed band size: 58-150 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721507) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CEACAM1 antibody (HA721507) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721507) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CEACAM1 antibody (HA721507) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721507) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.