CENPF Recombinant Rabbit Monoclonal Antibody [PSH0-79]
cat.: HA721508
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH0-79 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 358 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CENPF aa 2,825-3,114 / 3,114. |
| Positive control: | SK-Br-3 cell lysate, Jurkat cell lysate, HeLa cell lysates, human testis tissue, human tonsil tissue. |
| Subcellular location: | Cytoplasm, perinuclear region, Nucleus matrix, Chromosome, centromere, kinetochore, Cytoplasm, cytoskeleton, spindle. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P49454 Human |
| Alternative names: | AH antigen Cell cycle dependent 350K nuclear protein CENF CENP F CENP F kinetochore protein CENP-F CENPF CENPF kinetochore protein CENPF_HUMAN Centromere protein F 350/400ka Centromere protein F Centromere protein F, 350/400kDa CILD31 Hcp 1 Hcp1 Kinetochore protein CENP F Kinetochore protein CENPF Mitosin PRO1779 STROMS |
Images
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Fig1:
Western blot analysis of CENPF on different lysates with Rabbit anti-CENPF antibody (HA721508) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 358 kDa Observed band size: 358 kDa Exposure time: 3 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721508) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of CENPF on HeLa cell lysates with Rabbit anti-CENPF antibody (HA721508) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 358 kDa Observed band size: 358 kDa Exposure time: 2 minutes; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721508) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-CENPF antibody (HA721508) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721508) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CENPF antibody (HA721508) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721508) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.