Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | PSH0-81 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 125 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human PERK aa 51-450 / 1,116. |
Positive control: | MCF7 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, HeLa cell lysate, A549 cell lysate, U-87 MG cell lysate, K-562 cell lysate. |
Subcellular location: | Endoplasmic reticulum membrane. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: Q9NZJ5 Human |
Alternative names: | DKFZp781H1925 E2AK3_HUMAN EC 2.7.11.1 Eif2ak3 Eukaryotic translation initiation factor 2 alpha kinase 3 Eukaryotic translation initiation factor 2-alpha kinase 3 Heme regulated EIF2 alpha kinase HRI HsPEK Pancreatic eIF2 alpha kinase Pancreatic eIF2-alpha kinase PEK PRKR like endoplasmic reticulum kinase PRKR-like endoplasmic reticulum kinase WRS |
Fig1:
Western blot analysis of PERK on different lysates with Rabbit anti-PERK antibody (HA721510) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: HEK-293 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: A549 cell lysate (20 µg/Lane) Lane 6: U-87 MG cell lysate (20 µg/Lane) Lane 7: K-562 cell lysate (16 µg/Lane) Predicted band size: 125 kDa Observed band size: 140 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721510) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |