Lipoamide Dehydrogenase Recombinant Rabbit Monoclonal Antibody [PSH0-83]
cat.: HA721512
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH0-83 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Lipoamide Dehydrogenase 21-509 / 509. |
| Positive control: | Jurkat cell lysate, HeLa cell lysate, 293T cell lysate, MCF7 cell lysate, K-562 cell lysate, SK-Br-3 cell lysate, U-937 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, human colon tissue, human colon carcinoma tissue, HeLa. |
| Subcellular location: | Mitochondrion matrix, Nucleus, Cell projection, cilium, flagellum, Cytoplasmic vesicle, secretory vesicle, acrosome. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:5,000 1:200 |
| Uniprot #: | SwissProt: P09622 Human | O08749 Mouse |
| Alternative names: | Dehydrogenase complex, E3 component Diaphorase Dihydrolipoamide dehydrogenase Dihydrolipoyl dehydrogenase Dihydrolipoyl dehydrogenase mitochondrial dld DLDD DLDH DLDH_HUMAN E3 E3 branched chain aplha-keto acid E3 component of pyruvate dehydrogenase E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex GCSL Glycine cleavage system L protein Glycine cleavage system protein L LAD lipoamide dehydrogenase Lipoamide reductase Lipoyl dehydrogenase mitochondrial OTTHUMP00000206744 OTTHUMP00000206746 OTTHUMP00000206748 OTTHUMP00000206749 PHE 3 PHE3 Pyruvate dehydrogenase component E3 |
Images
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Fig1:
Western blot analysis of Lipoamide Dehydrogenase on different lysates with Rabbit anti-Lipoamide Dehydrogenase antibody (HA721512) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: MCF7 cell lysate (20 µg/Lane) Lane 5: K-562 cell lysate (20 µg/Lane) Lane 6: SK-Br-3 cell lysate (20 µg/Lane) Lane 7: U-937 cell lysate (20 µg/Lane) Lane 8: HepG2 cell lysate (20 µg/Lane) Lane 9: NIH/3T3 cell lysate (20 µg/Lane) Lane 10: Mouse liver tissue lysate (40 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721512) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Lipoamide Dehydrogenase antibody (HA721512) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721512) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Lipoamide Dehydrogenase antibody (HA721512) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721512) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of HeLa cells labeling Lipoamide Dehydrogenase with Rabbit anti-Lipoamide Dehydrogenase antibody (HA721512) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Lipoamide Dehydrogenase antibody (HA721512) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.