Phospho-Rb (S780) Recombinant Rabbit Monoclonal Antibody [JE44-61]
cat.: HA721513
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE44-61 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 106 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to resudues surrounding Ser780 of human Rb protein. |
| Positive control: | Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours whole cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P06400 Human | P13405 Mouse | P33568 Rat |
| Alternative names: | Exon 17 tumor GOS561 substitution mutation causes premature stop GOS563 exon 17 substitution mutation causes premature stop OSRC Osteosarcoma p105-Rb P105RB PP105 pp110 PPP1R130 pRb Prepro retinoblastoma associated protein Protein phosphatase 1 regulatory subunit 130 Rb RB transcriptional corepressor 1 RB_HUMAN RB1 RB1 gene Retinoblastoma 1 Retinoblastoma suspectibility protein Retinoblastoma-associated protein |
Images
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Fig1:
Western blot analysis of Phospho-Rb (S780) on different lysates with Rabbit anti-Phospho-Rb (S780) antibody (HA721513) at 1/1,000 dilution. Lane 1: Jurkat serum starved for 44 hours whole cell lysate Lane 2: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours whole cell lysate Lane 3: Jurkat serum starved for 44 hours then treated with λpp for 1 hour whole cell lysate Lane 4: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours then treated with λpp for 1 hour whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 106 kDa Observed band size: 106 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721513) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Phospho-Rb (S780) on NIH/3T3 cell lysates with Rabbit anti-Phospho-Rb (S780) antibody (HA721513) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 106 kDa Observed band size: 106 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721513) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.