Phospho-Rb (S780) Recombinant Rabbit Monoclonal Antibody [JE44-61]
cat.: HA721513
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE44-61
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 106 kDa
Isotype: IgG
Immunogen: Synthetic phosphopeptide corresponding to resudues surrounding Ser780 of human Rb protein.
Positive control: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours whole cell lysate.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P06400 Human | P13405 Mouse | P33568 Rat
Alternative names: Exon 17 tumor GOS561 substitution mutation causes premature stop GOS563 exon 17 substitution mutation causes premature stop OSRC Osteosarcoma p105-Rb P105RB PP105 pp110 PPP1R130 pRb Prepro retinoblastoma associated protein Protein phosphatase 1 regulatory subunit 130 Rb RB transcriptional corepressor 1 RB_HUMAN RB1 RB1 gene Retinoblastoma 1 Retinoblastoma suspectibility protein Retinoblastoma-associated protein
Images
HA721513_1.jpg Fig1: Western blot analysis of Phospho-Rb (S780) on different lysates with Rabbit anti-Phospho-Rb (S780) antibody (HA721513) at 1/1,000 dilution.

Lane 1: Jurkat serum starved for 44 hours whole cell lysate
Lane 2: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours whole cell lysate
Lane 3: Jurkat serum starved for 44 hours then treated with λpp for 1 hour whole cell lysate
Lane 4: Jurkat serum starved for 24 hours then 10% FBS incubated for 20 hours then treated with λpp for 1 hour whole cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 106 kDa
Observed band size: 106 kDa

Exposure time: 1 minute 59 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721513) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.