c-Myb Recombinant Rabbit Monoclonal Antibody [JE40-07]
cat.: HA721514
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE40-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 72 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human c-Myb aa 591-640 / 640. |
| Positive control: | Jurkat cell lysate, HL-60 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P10242 Human |
| Alternative names: | C myb c myb protein (140 AA) c-myb protein (140 AA) c-myb_CDS c-myb10A_CDS c-myb13A_CDS c-myb14A_CDS c-myb8B_CDS Cmyb efg Myb MYB proto-oncogene transcription factor MYB_HUMAN ONCOGENE AMV Oncogene MYB Proto oncogene c Myb Proto-oncogene c-Myb Transcriptional activator Myb v myb avian myeloblastosis viral oncogene homolog v-myb avian myeloblastosis viral oncogene homolog |
Images
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Fig1:
Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (HA721514) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: 3 minutes 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721514) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.