Pancreatic Polypeptide Recombinant Rabbit Monoclonal Antibody [PSH0-84]
cat.: HA721515
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-P, IHC-Fr, IF-Tissue, mIHC |
| Clonality: | Monoclonal |
| Clone number: | PSH0-84 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 10 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PPY aa 30-95. |
| Positive control: | Mouse pancreas tissue, rat pancreas tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P IHC-Fr IF-Tissue mIHC |
1:1,000 1:500 1:500 1:2,000 |
| Uniprot #: | SwissProt: P10601 Mouse | P06303 Rat |
| Alternative names: | Pancreatic polypeptide Pancreatic polypeptide Y Pancreatic prohormone PNP PP PPY prepro-PP (prepropancreatic polypeptide) |
Images
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Fig1:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Pancreas Sample: Frozen section Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in TBST, 10 minutes at room temperature. Primary antibody: Pancreatic Polypeptide (HA721515, 1/500, Green), Insulin (EM80714, 1/500, Red), overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), Goat anti-Mouse IgG (iFluor™ 594, HA1126), 1.5 hours at room temperature. |
|
Fig2:
Application: Immunofluorescence (IHC-Fr) Species: Rat Tissue: Pancreas Sample: Frozen section Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in TBST, 10 minutes at room temperature. Primary antibody: Pancreatic Polypeptide (HA721515, 1/500, Green), Insulin (EM80714, 1/500, Red), overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), Goat anti-Mouse IgG (iFluor™ 594, HA1126), 1.5 hours at room temperature. |
|
Fig3:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Liver Sample: Frozen section Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in TBST, 10 minutes at room temperature. Primary antibody: HA721515, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Pancreatic Polypeptide antibody (HA721515) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721515) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Pancreatic Polypeptide antibody (HA721515) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721515) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Application: Immunofluorescence (IF-tissue) Species: Mouse Tissue: pancreas Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBS Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA721515, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.