Phospho-Acetyl Coenzyme A Carboxylase (S79) Recombinant Rabbit Monoclonal Antibody [JE55-54]
cat.: HA721516
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE55-54 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 277 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Ser79 of human acetyl-CoA carboxylase protein. |
| Positive control: | SH-SY5Y treated with 0.5μM Oligomycin for 30 minutes whole cell lysate. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q13085 Human | Q5SWU9 Mouse | P11497 Rat |
| Alternative names: | ACAC ACACA ACACA_HUMAN ACC alpha ACC ACC-alpha ACC1 ACCA Acetyl CoA carboxylase 1 Acetyl CoA carboxylase alpha Acetyl Coenzyme A carboxylase alpha Biotin carboxylase COA1 HACC275 OTTHUMP00000164069 OTTHUMP00000164070 OTTHUMP00000164076 OTTHUMP00000240532 |
Images
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Fig1:
Western blot analysis of Phospho-Acetyl Coenzyme A Carboxylase (S79) on different lysates with Rabbit anti-Phospho-Acetyl Coenzyme A Carboxylase (S79) antibody (HA721516) at 1/1,000 dilution. Lane 1: SH-SY5Y whole cell lysate Lane 2: SH-SY5Y treated with 0.5μM Oligomycin for 30 minutes whole cell lysate Lane 3: SH-SY5Y treated with λpp for 1 hour whole cell lysate Lane 4: SH-SY5Y treated with 0.5μM Oligomycin for 30 minutes then treated with λpp for 1 hour whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 277 kDa Observed band size: 277 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721516) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.