HA721520

F4/80 Recombinant Rabbit Monoclonal Antibody [PSH0-85]

cat.: HA721520

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Mouse, Rat
Applications:	WB, IHC-P, IF-Tissue, IHC-Fr, mIHC
Clonality:	Monoclonal
Clone number:	PSH0-85

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 102 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within mouse F4/80 aa 1-650 / 931.
Positive control:	Mouse kidney tissue lysate, RAW264.7 cell lysate, Rat kidney tissue lysate, Rat spleen tissue lysate, mouse liver tissue, mouse spleen tissue, rat liver tissue, rat spleen tissue, mouse lung tissue, rat lung tissue.
Subcellular location:	Cell membrane.
Recommended Dilutions: WB IHC-P IF-Tissue IHC-Fr mIHC	1:1,000 1:200-1:1,000 1:500 1:1,000 1:500
Uniprot #:	SwissProt: Q61549 Mouse \| Q5Y4N8 Rat
Alternative names:	ADGRE1 Adhesion G protein coupled receptor E1 Adhesion G protein-coupled receptor E1 AGRE1_HUMAN Cell surface glycoprotein EMR1 Cell surface glycoprotein F4/80 DD7A5 7 Egf like module containing mucin like hormone receptor like 1 Egf like module containing mucin like hormone receptor like sequence 1 EGF like module receptor 1 EGF TM7 EGF-like module receptor 1 EGF-like module-containing mucin-like hormone receptor-like 1 EGFTM7 EMR 1 EMR1 EMR1 hormone receptor Gpf480 Ly71 Lymphocyte antigen 71 TM7LN3

Images

Fig1: Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution.

Lane 1: Mouse kidney tissue lysate (no heat) (40 µg/Lane)
Lane 2: Mouse kidney tissue lysate (40 µg/Lane)
Lane 3: RAW264.7 cell lysate (no heat) (20 µg/Lane)
Lane 4: Rat kidney tissue lysate (no heat) (40 µg/Lane)

Notice: no heat means the lysate is not boiled.

Predicted band size: 102 kDa
Observed band size: 160 kDa

Exposure time: 59 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721520) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution.

Lane 1: RAW264.7 cell lysate (no heat) (20 µg/Lane)
Lane 2: L929 cell lysate (no heat) (negative) (20 µg/Lane)
Lane 3: Rat spleen tissue lysate (70℃ heat) (40 µg/Lane)

Notice: no heat means the lysate is not boiled.

Predicted band size: 102 kDa
Observed band size: 160 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721520) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

	Fig3: Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 (F4/80, HA721520, rabbit, red); 1/500 (Iba1, HA601376, Chicken, green) Antigen retrieval: Not required
	Fig4: Application: IHC-Fr Species: Rat Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 (F4/80, HA721520, rabbit, red); 1/500 (Iba1, HA601376, Chicken, green) Antigen retrieval: Not required
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721520) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721520) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721520) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig8: Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721520) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig9: Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721520) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig10: Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-F4/80 antibody (HA721520) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721520) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig11: Application: IF-tissue Species: Mouse Site: Liver Sample: Paraffin-embedded section Antibody concentration: 1/500

	Fig12: Application: IF-tissue Species: Rat Site: Liver Sample: Paraffin-embedded section Antibody concentration: 1/500
	Fig13: Application: IF-tissue Species: Mouse Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/500
	Fig14: Application: IF-tissue Species: Mouse Site: small intestine Sample: Paraffin-embedded section Antibody concentration: 1/500
	Fig15: mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-F4/80 antibody (HA721520) at 1/500 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.