ERCC8 Recombinant Rabbit Monoclonal Antibody [PSH0-86]
cat.: HA721521
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: PSH0-86
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 44 kDa
Isotype: IgG
Immunogen: Recombinant protein within human ERCC8 aa 1-396 / 396.
Positive control: 293T cell lysate, SH-SY5Y cell lysate, HepG2 cell lysate.
Subcellular location: Nucleus, Nucleus matrix.
Recommended Dilutions:
  WB

1:1,000-1:5,000
Uniprot #: SwissProt: Q13216 Human
Alternative names: CKN1 Cockayne syndrome type A Cockayne syndrome WD repeat protein CSA CSA DNA excision repair protein ERCC-8 DNA excision repair protein ERCC8 ERCC 8 ERCC8 ERCC8_HUMAN excision repair cross-complementing rodent repair deficiency, complementation group 8
Images
HA721521_1.jpg Fig1: Western blot analysis of ERCC8 on different lysates with Rabbit anti-ERCC8 antibody (HA721521) at 1/1,000 dilution.

Lane 1: 293T cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: HepG2 cell lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 44 kDa
Observed band size: 55 kDa

Exposure time: 27 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721521) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721521_2.jpg Fig2: Western blot analysis of ERCC8 on different lysates with Rabbit anti-ERCC8 antibody (HA721521) at 1/5,000 dilution.

Lane 1: 293T-si NT cell lysate
Lane 2: 293T-si ERCC8 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 44 kDa
Observed band size: 55 kDa

Exposure time: 11 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721521) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.