ERCC8 Recombinant Rabbit Monoclonal Antibody [PSH0-86]
cat.: HA721521
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH0-86 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ERCC8 aa 1-396 / 396. |
| Positive control: | 293T cell lysate, SH-SY5Y cell lysate, HepG2 cell lysate. |
| Subcellular location: | Nucleus, Nucleus matrix. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: Q13216 Human |
| Alternative names: | CKN1 Cockayne syndrome type A Cockayne syndrome WD repeat protein CSA CSA DNA excision repair protein ERCC-8 DNA excision repair protein ERCC8 ERCC 8 ERCC8 ERCC8_HUMAN excision repair cross-complementing rodent repair deficiency, complementation group 8 |
Images
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Fig1:
Western blot analysis of ERCC8 on different lysates with Rabbit anti-ERCC8 antibody (HA721521) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 44 kDa Observed band size: 55 kDa Exposure time: 27 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721521) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ERCC8 on different lysates with Rabbit anti-ERCC8 antibody (HA721521) at 1/5,000 dilution. Lane 1: 293T-si NT cell lysate Lane 2: 293T-si ERCC8 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 44 kDa Observed band size: 55 kDa Exposure time: 11 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721521) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.